The return flight would depart from Mangaluru at 12.55 PM and arrive at Mysuru at 1.55 PM, the release said.
The non-UDAN flight service would be functional on Wednesdays, Fridays, Saturdays and Sundays, it said.
UDAN (Ude Desh Ka Aam Nagrik) is a regional connectivity scheme launched by the Central government with a vision to make air travel affordable.
The connectivity between Mangaluru and Mysuru is significant as the latter is home to nearly 65,000 people or around 12,000 families and also a number of entrepreneurs having business interests across the two regions.
The new flight service has been welcomed by stakeholders from the tourism and hospitality sectors and various trade bodies.