"Very professional staff is needed to find targets and fire this missile," he said in an interview to be published on Sunday in Frankfurter Allgemeine Sonntagszeitung.
"It is possible that these people came from Russia."
Referring to the possibility that the missile was launched by pro-Russian separatists who have been fighting Kiev, he said "(It could) not be operated by drunken gorillas".
Kiev raised the stakes on Saturday by saying it had evidence that a Russian fired the missile widely assumed to have brought down the plane in east Ukraine last Thursday.
Yatseniuk ruled out that the missile could have been launched by Ukraine. "All of Ukraine's ground-air-missiles are stationed elsewhere. We are ready to make public evidence and locations."