Fans or paparazzi will face a 400 pounds fine for loitering round, stopping at or even swimming up to the home on Lake Como until September 30, reported New York Daily News online.
Clooney, 53, plans to marry human rights lawyer Amal Alamuddin, 36, that month but had said he did not want to annoy his neighbours.
The laws announced by Laglio Council are in operation 24 hours a day until September 30. Notices have been stuck on lampposts in the area with a warning in red type.
Laglio mayor Roberto Pozzi said that in the past there had been 'unpleasant episodes' when fans had gone up to Clooney's windows and yelled out his name.
The new rules were designed to stop them doing the same and to prevent paparazzi from getting too close to his home, he said.
"It is now completely forbidden to stop outside the entrance to Clooney's villa. It is a question of traffic and security. It is very dangerous and it's also an issue of privacy," Pozzi said.
Clooney is currently making the most of his Italian mansion and has been taking his fiancee on a number of romantic dates in the area.
He also flew her mother Baria Alamuddin, 66, out to spend time with them over the weekend in what appears to be their first meeting together.
Clooney and Amal, who is of British-Lebanese origin, have been engaged since April following a whirlwind romance when they met at a charity event in September.