FlipKart did not disclose its new holding pattern.
The sources said, however, that with this round of fund raising, FlipKart is valued at about USD 7 billion (around Rs 42,000 crore).
Co-led by existing investors Tiger Global Management and Naspers, Singapore's sovereign wealth fund, GIC, Accel Partners, DST Global, ICONIQ Capital, Morgan Stanley Investment Management and Sofina also participated in this latest financing round.
The Bangalore-based firm will utilise funds on expanding its online and mobile services, focusing on areas like R&D, enhancing customer experience and sellerbase.
Flush with cash, FlipKart is also scouting for acquisitions, which can help it expand into newer technologies like wearables and robotics, a move that it believes will impact mobile commerce in the days to come.
"The funds will be used to make long-term strategic investments in India, especially in mobile technology," FlipKart co-founder and CEO Sachin Bansal told reporters here.
The focus at FlipKart is to continue to make shopping online simpler and more accessible through the use of technology, he added.
"This funding will enable us to step up our investments for innovations in products and technologies, setting us up to become the mobile e-commerce company of the future. This funding will help us further accelerate momentum and build our presence to become a technology powerhouse," he said.
On the company's IPO plans, Bansal said: "IPO is not in consideration at all, we are not thinking about it. We have not settled on a business model that we can take public."
In May, FlipKart had raised USD 210 million funding, bringing private equity firm DST Global on board as an investor.
It is estimated that the firm has, so far, raised over USD 1.7 billion from investors, including the current transaction.
The Bangalore-based firm, founded by Sachin Bansal and Binny Bansal, counts Accel Partners, Dragoneer Investment Group, Morgan Stanley Investment Management, Sofina and Vulcan Capital among its other investors.
The home-grown e-retailer had acquired online fashion retailer Myntra in May in what is estimated to be a Rs 2,000-crore deal.
It had also announced an investment of USD 100 million (around Rs 600 crore) in its fashion business over the next 12-18 months.
Flipkart, currently 14,000 people strong, has 22 million registered users clocking over 4 million daily visits. It delivers 5 million shipments per month, which the company claims is growing rapidly.
Flipkart's moves are being seen as efforts to protect its turf in the USD 3 billion Indian e-commerce market that is witnessing aggressive competition from global giant Amazon and peers like Snapdeal.
Led by increasing Internet penetration and youngsters shopping online, India's e-commerce market has seen huge growth in the past few years. As more people log on to the Internet to shop, it is estimated to expand over seven-fold to USD 22 billion by 2018.
Flipkart had a 4.9 per cent market share in 2013, while Amazon and eBay had 1.6 per cent and 1.2 per cent share respectively.
Flipkart, which started in 2007 as an online bookstore, sells products across categories, including fashion and electronics. It also sells white goods and furniture.
While apparel and electronics are bestsellers for most e-commerce firms, categories such as home decor and household items are also popular.
"We believe the Internet will improve the quality of life for millions of Indians, and e-commerce is going to play a huge role in this change," Bansal said.
The company also plans to hire 1,000 engineers with an eye on expanding its R&D capabilities and is also looking at roping in mobile and technology experts from Silicon Valley.
"By 2020, India will have more than half a billion mobile Internet users. Our intense focus on mobile and technology puts us in a unique position to take advantage of this massive opportunity," Bansal said.