India is the most prevalent among a group of developing nations angry at rich countries for failing to address their concerns about a deal on trade facilitation - struck by WTO member states in Bali last year - that must be detailed by a July 31 deadline.
Proponents believe the deal could add $1 trillion to global GDP and 21 million jobs.
But Indias Trade Ministry said on Wednesday it would find it difficult" to support the protocol unless it was satisfied that adequate emphasis is being placed on negotiations about food security and other issues important to poor countries -sparking furious negotiations at the G20 Trade Ministers meeting in Sydney.
Three officials involved in the negotiations, speaking under the condition of anonymity in order to speak frankly, expressed exasperation with what they described as a history of erratic behaviour on the part of the Indian trade team that made it difficult to trust.
India has not provided any clear description of exactly what changes it would like made to the agreement, they said, although it would not matter anyway because no concessions were on offer given how difficult the negotiations had proven to conclude the first time round.
The Indian demands appear to have shaken confidence in the new government of Narendra Modi, who came to power earlier this year with a pro-business agenda but now appears set to derail what several officials called the most significant global trade pact in two decades.
Australian Trade Minister Andrew Robb said assurances had been given to all of the signatories to the treaty that their concerns would be met and expressed optimism that it would be resolved before the deadline.
"There was strong resolution around the table that Indias issues to do with food security would and should and will be addressed as decided previously and I think there will be discussions about how to satisfy the Indians and they wont be left behind," Robb told reporters.
The row over subsidies has raised fears that the so-called "trade facilitation agreement", the first ever global trade agreement under the World Trade Organization, will be derailed.
A deal was only reached after New Delhi extracted promises that its concerns related to food subsidies would be addressed.
India stockpiles food for its poor, putting it at risk of breaking current WTO rules. In Bali, WTO members agreed to give India a pass until 2017, while negotiating a permanent solution.
"We are focused on implementing the full Bali package that will deliver for every country involved," said Michael Froman, the U.S. Trade Representative."Reinvigorating the multilateral system is too important to put at risk with any backsliding on commitments."