All four existing investors Greylock Partners, Lightspeed Venture Partners, Kleiner Perkins Caufield & Byers and Institutional Venture Partners also participated in the round.
The funding also includes USD 50 million in debt financing provided by Silicon Valley Bank.
The new round brings the total capital raised to over USD 200 million and will fund its expansion of Application Intelligence Platform into IT operations and business analytics markets and also accelerate go-to-market investments worldwide, AppDynamics said in a statement.
"This financing will help fuel our continued rapid pace of technology innovation, and expansion of our worldwide sales and go-to-market execution," AppDynamics founder and CEO Jyoti Bansal said.
Application intelligence is rapidly becoming the foundational management platform for modern enterprises that are going through a digital transformation, he added.
It has the opportunity to reshape not only the USD 4-5 billion per year application performance management market, but the broader USD 20 billion per year IT operations management and USD 34 billion per year operations and business analytics markets, Bansal said.
Its application intelligence platform helps software defined businesses monitor, manage and optimise the most complex software environments, all in real time and all in production.
Its customers include Citrix, Edmunds, Expedia, Fox News, John Deere, OpenTable, Salesforce, Sephora, StubHub and Union Pacific Railroad.