Starring Jamie Dornan and Dakota Johnson, the teaser has become the most-watched of 2014, according to technology firm Zefr, said the Hollywood Reporter.
The previous high was set by 'Teenage Mutant Ninja Turtles', which racked up 31 million views.
The 'Fifty Shades' book series by EL James has sold more than 100 million copies worldwide, and been translated into 52 different languages.
'Fifty Shades of Grey's supporting cast features Rita Ora, Marcia Gay Harden, Max Martini, Eloise Mumford and Jennifer Ehle.
The film, directed by 'Nowhere Boy' helmer Sam Taylor-Johnson, will be released in cinemas on Valentine's Day 2015.